What happens in cells (and what do cells need)?

EasyMediumHard
123456789101112131415161718192021222324252627282930313233
Question 30
Medium

In research laboratories, scientists can synthesize specific proteins in cell-free systems using in vitro translation kits.

These kits typically contain:

  • A DNA template containing the gene of interest
  • Free RNA nucleotides
  • Ribosomes, tRNA molecules, enzymes, amino acids, and an energy source (ATP)
a.

Describe the role of the DNA template and free RNA nucleotides during the transcription phase in this kit.

[2]
b.

Describe the role of tRNA molecules and ribosomes during the translation phase in this kit.

[2]
c.

Polymerase Chain Reaction (PCR) is a molecular biology technique used to amplify DNA.

Explain how PCR enables scientists to analyze extremely small or historic DNA samples found at ancient archaeological sites.

[2]
d.

Name the specific phase of the eukaryotic cell cycle that performs the same biochemical function as PCR.

[1]
e.

Genetic databases store DNA profiles of individuals.

Suggest two benefits of utilizing genetic databases in modern healthcare, other than solving crimes.

[2]
f.

State one ethical concern a person might have regarding the storage of their genetic profile on a database.

[1]

What happens in cells (and what do cells need)? Questions

  1. GCSE
  2. /Biology
  3. /What happens in cells (and what do cells need)?