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1.2 What happens in cells (and what do cells need)?

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Question 23
H

A student investigates the effect of temperature on the activity of amylase.

  • She mixes amylase solution and starch solution in a water bath.
  • Every 30 seconds she tests a drop of the mixture with iodine solution.
  • She records the first time at which the iodine solution does not turn blue-black.

The Table shows her results.

Table

Temperature in ∘C^\circ\text{C}∘CTime until starch is no longer present in s\text{s}s
20270
30120
4060
5060
60still blue-black after 600
a.

The rate of reaction can be calculated using this equation:

rate=1000time\text{rate} = \dfrac{1000}{\text{time}}rate=time1000​

Calculate the rate of reaction at 30 ∘C30\ ^\circ\text{C}30 ∘C.

Give your answer to 2 significant figures.

[1]
b.

The student concludes:

'The optimum temperature for amylase is 40 ∘C40\ ^\circ\text{C}40 ∘C.'

Evaluate this conclusion.

[3]
c.

Suggest two changes to the method that would allow the student to find the optimum temperature more precisely.

[2]
d.

Explain why the iodine solution still turned blue-black after 600 seconds at 60 ∘C60\ ^\circ\text{C}60 ∘C.

[2]
Markscheme

1.2 What happens in cells (and what do cells need)? Questions

  1. GCSE
  2. /Biology
  3. /1.2 What happens in cells (and what do cells need)?

184 exam-style questions on OCR GCSE Biology 1.2 What happens in cells (and what do cells need)?, covering 1.2.1 Structure of DNA, 1.2.2 Nucleotides and complementary bases, 1.2.3 Protein synthesis, 1.2.4 DNA structure and the proteins made, 1.2.5 Investigating enzymatic reactions, and 1.2.6 Mechanism of enzyme action. Each one has a worked solution and a mark scheme showing where the marks go.

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