A student boils some catalase solution and then cools it to 25 ∘C25\ ^\circ\text{C}25 ∘C.
The student adds the cooled solution to hydrogen peroxide. No bubbles of oxygen are produced.
Which statement explains this?
Cooling slowed the enzyme down, so it needs more time to react.
Boiling changed the shape of the active site permanently.
Boiling killed the enzyme, so it can no longer move or react.
Cooling made the substrate too cold to react with the enzyme.
184 exam-style questions on OCR GCSE Biology 1.2 What happens in cells (and what do cells need)?, covering 1.2.1 Structure of DNA, 1.2.2 Nucleotides and complementary bases, 1.2.3 Protein synthesis, 1.2.4 DNA structure and the proteins made, 1.2.5 Investigating enzymatic reactions, and 1.2.6 Mechanism of enzyme action. Each one has a worked solution and a mark scheme showing where the marks go.