Tay-Sachs disease is a rare lysosomal storage disorder characterized by the dysfunctional breakdown of GM2 gangliosides, causing these lipids to accumulate to toxic levels inside neurons. If a neuropathologist extracts these accumulated biomolecules from a cellular sample, which biochemical test and subsequent observation would successfully confirm the presence of lipids in the extract?
Mix with iodine in potassium iodide solution; observation of a deep blue-black coloration.
Add Benedict's reagent and heat in a water bath; formation of a brick-red precipitate.
Shake the sample with absolute ethanol, then pour into water; formation of a cloudy white emulsion.
Add Biuret reagent (sodium hydroxide and copper(II) sulfate); color change from light blue to purple.
72 exam-style questions on OCR GCSE Biology 1.2 What happens in cells (and what do cells need)?, covering 1.2.1 Structure of DNA, 1.2.2 Nucleotides and complementary bases, 1.2.3 Protein synthesis, 1.2.4 DNA structure and the proteins made, 1.2.5 Investigating enzymatic reactions, and 1.2.6 Mechanism of enzyme action. Each one has a worked solution and a mark scheme showing where the marks go.