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1.2 What happens in cells (and what do cells need)?

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Question 11

Two samples of a human protease are kept for 10 minutes at different temperatures, with no substrate present.

Sample R is kept at 15 ∘C15\ ^\circ\text{C}15 ∘C and sample S is kept at 65 ∘C65\ ^\circ\text{C}65 ∘C. Both samples are then brought to 37 ∘C37\ ^\circ\text{C}37 ∘C before equal amounts of protein are added.

Protein is broken down in sample R but not in sample S.

Which statement explains this?

[1]
A

Protease in sample S shifted its optimum to 65 ∘C65\ ^\circ\text{C}65 ∘C, so 37 ∘C37\ ^\circ\text{C}37 ∘C was too cold for it to work.

B

Protease in sample S kept extra kinetic energy from 65 ∘C65\ ^\circ\text{C}65 ∘C, so it moved too fast to bind protein.

C

Protease in sample R retained its active-site shape, whereas protease in sample S was denatured at 65 ∘C65\ ^\circ\text{C}65 ∘C.

D

Protease in sample S was used up at 65 ∘C65\ ^\circ\text{C}65 ∘C, whereas protease in sample R was not used up at 15 ∘C15\ ^\circ\text{C}15 ∘C.

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1.2 What happens in cells (and what do cells need)? Questions

  1. GCSE
  2. /Biology
  3. /1.2 What happens in cells (and what do cells need)?

184 exam-style questions on OCR GCSE Biology 1.2 What happens in cells (and what do cells need)?, covering 1.2.1 Structure of DNA, 1.2.2 Nucleotides and complementary bases, 1.2.3 Protein synthesis, 1.2.4 DNA structure and the proteins made, 1.2.5 Investigating enzymatic reactions, and 1.2.6 Mechanism of enzyme action. Each one has a worked solution and a mark scheme showing where the marks go.

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