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1.2 What happens in cells (and what do cells need)?

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Question 10

A student investigates how pH affects catalase in potato.

For each pH value, she:

  • places equal masses of potato in the same volume and concentration of hydrogen peroxide
  • uses a buffer solution to control the pH
  • keeps the mixture at 30 ∘C30\ ^\circ\text{C}30 ∘C
  • collects the oxygen produced in 60 seconds using a gas syringe.

She tests each pH value once.

pHVolume of oxygen collected in 60 s in cm3\text{cm}^3cm3
30.6
614.4
718.0
816.8
a.

The rate of oxygen production is found using:

rate=volume of oxygentime\text{rate} = \dfrac{\text{volume of oxygen}}{\text{time}}rate=timevolume of oxygen​

Calculate the rate at pH 6.

Write your answer in cm3 s−1\text{cm}^3\,\text{s}^{-1}cm3s−1 to 2 significant figures.

[1]
b.

The student uses the results to claim that the optimum pH of the catalase is exactly pH 7.

Evaluate this claim.

[3]
c.

Suggest two modifications that would provide stronger evidence for the optimum pH.

[2]
d.

Only 0.6 cm3 of oxygen was collected at pH 3.

Explain this result.

[2]
Markscheme

1.2 What happens in cells (and what do cells need)? Questions

  1. GCSE
  2. /Biology
  3. /1.2 What happens in cells (and what do cells need)?

184 exam-style questions on OCR GCSE Biology 1.2 What happens in cells (and what do cells need)?, covering 1.2.1 Structure of DNA, 1.2.2 Nucleotides and complementary bases, 1.2.3 Protein synthesis, 1.2.4 DNA structure and the proteins made, 1.2.5 Investigating enzymatic reactions, and 1.2.6 Mechanism of enzyme action. Each one has a worked solution and a mark scheme showing where the marks go.

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