A student used a colorimeter to monitor an enzyme-controlled reaction where a substrate is converted into a colored product.
Table 1 shows the absorbance measurements recorded during the reaction.
| Volume of reaction mixture in cm3\text{cm}^3cm3 | Initial absorbance | Final absorbance |
|---|---|---|
| 15 | 0.18 | 0.68 |
Calculate the mass of product formed during this reaction.
Use the equation:
mass of product in micrograms (μg)=volume of reaction mixture in cm3×8.6×change in absorbance \text{mass of product in micrograms } (\mu\text{g}) = \text{volume of reaction mixture in } \text{cm}^3 \times 8.6 \times \text{change in absorbance} mass of product in micrograms (μg)=volume of reaction mixture in cm3×8.6×change in absorbance
