A student is investigating the effect of temperature on the rate of hydrolysis of starch by the enzyme amylase.
Before combining the enzyme and substrate, they place separate tubes containing the amylase solution and the starch solution into a water bath set at 40 ∘C40\ ^\circ\text{C}40 ∘C for 10 minutes.
State why it is necessary to incubate both solutions in the water bath before mixing them.
