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Enzymes

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Question 21

Catalase is an enzyme that catalyses the breakdown of hydrogen peroxide into water and oxygen gas.

A student designed an experiment to investigate the effect of hydrogen peroxide concentration on the rate of this catalase-controlled reaction.

The student had access to:

  • five boiling tubes, each containing a different concentration of hydrogen peroxide solution
  • a flask of pH 7 buffer solution
  • a beaker of yeast catalase enzyme suspension
  • gas syringes, delivery tubes, and rubber bungs.

The first step of the student's plan is:

Step 1. Add 2 cm3 of the pH 7 buffer solution to each of the five boiling tubes.

Describe the next two steps that should be in this plan to obtain valid results for this investigation.

[2]

Enzymes Questions

  1. GCSE
  2. /Biology
  3. /Enzymes