A student investigates the activity of the enzyme sucrase. They incubate a sample of sucrose with sucrase at 37∘C37^\circ\text{C}37∘C for 30 minutes. To determine whether the enzymatic hydrolysis of sucrose was successful, the student performs a biochemical test on a sample of the reaction mixture. Which of the following describes the correct test, observation, and explanation for a successful reaction?
Benedict's reagent is added and heated; the solution turns brick-red because the reducing sugars glucose and fructose have been produced.
Benedict's reagent is added and heated; the solution remains blue because sucrose is a non-reducing sugar that is unaffected by sucrase.
Iodine solution is added; the solution turns blue-black because the glycosidic bonds in sucrose have been converted into starch.
Biuret reagent is added and heated; the solution turns brick-red because sucrase is a protein enzyme.
