Bioluminescent marine bacteria contain an enzyme called bacterial luciferase.
This enzyme catalyses a reaction between a substrate called luciferin and dissolved oxygen to produce a blue-green light.
A student designed an experiment to investigate the effect of luciferin concentration on this bioluminescent reaction.
The student had access to:
The first step of the student's plan is:
Step 1. Add 5 cm3 of the oxygenated buffer solution to each of the five test tubes.
Describe the next two steps that should be in this plan to obtain valid results for this investigation.
