An investigation is carried out to determine the factors needed for photosynthesis using a healthy, green-leaved Pelargonium plant.
Before starting the investigation, the plant is kept in a dark room for 72 hours to destarch it.
Following this, two leaves on the plant are prepared as follows:
The plant is then placed under an intensive LED grow lamp for 18 hours. Both leaves are then detached and tested for the presence of starch using iodine solution.
Explain why the plant must be destarched before the investigation begins.
Predict whether the following regions of the leaves will turn blue-black or remain yellow-brown after the iodine test:
Describe how to safely remove chlorophyll from a leaf before testing it with iodine solution.