Question 21 – 2.2 Animal tissues, organs and organ systems - Questions

A scientist investigated the effect of a competitive amylase inhibitor, acarbose, on the rate of starch digestion by human pancreatic amylase.

In the experiment, the scientist:

Mixed $cm32\text{ cm}^3$ of $1%1\%$ starch solution with $cm31\text{ cm}^3$ of human pancreatic amylase solution.
Added $cm31\text{ cm}^3$ of acarbose solution at varying concentrations ( $0.0$ , $0.2$ , and $dm−30.5\text{ mmol dm}^{-3}$ ).
Maintained the mixtures at a constant temperature of $37∘C37^\circ\text{C}$ and a pH of $7.0$ .
Extracted samples every 5 seconds and added them to iodine solution in a spotting tile to find the exact time when starch was completely digested.

The investigation was repeated three times for each concentration of the inhibitor. The results are presented in the table below:

Concentration of acarbose ( $dm−3\text{mmol dm}^{-3}$ )	Test 1 (s)	Test 2 (s)	Test 3 (s)	Mean (s)
0.0	46	51	47	48
0.2	114	122	118	118
0.5	267	278	271	W

A scientist investigated the effect of a competitive amylase inhibitor, acarbose, on the rate of starch digestion by human pancreatic amylase.

In the experiment, the scientist:

Mixed $cm32\text{ cm}^3$ of $1%1\%$ starch solution with $cm31\text{ cm}^3$ of human pancreatic amylase solution.
Added $cm31\text{ cm}^3$ of acarbose solution at varying concentrations ( $0.0$ , $0.2$ , and $dm−30.5\text{ mmol dm}^{-3}$ ).
Maintained the mixtures at a constant temperature of $37∘C37^\circ\text{C}$ and a pH of $7.0$ .
Extracted samples every 5 seconds and added them to iodine solution in a spotting tile to find the exact time when starch was completely digested.

The investigation was repeated three times for each concentration of the inhibitor. The results are presented in the table below:

Concentration of acarbose ( $dm−3\text{mmol dm}^{-3}$ )	Test 1 (s)	Test 2 (s)	Test 3 (s)	Mean (s)
0.0	46	51	47	48
0.2	114	122	118	118
0.5	267	278	271	W

2.2 Animal tissues, organs and organ systems