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Methods of separating and purifying substances

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Question 22

A student is separating the photosynthetic pigments from a spinach leaf extract using thin-layer chromatography (TLC). After spotting the extract on the silica gel plate, they place it inside a beaker containing a mobile phase of petroleum ether and acetone. The student ensures that the beaker is sealed with a watch glass on top.

Explain why placing a lid on the beaker is necessary for the chromatography to run successfully.

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Methods of separating and purifying substances Questions

  1. GCSE
  2. /Chemistry
  3. /Methods of separating and purifying substances