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Antibiotics, culturing microorganisms and monoclonal antibodies

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Question 55

Before a new antimicrobial wound spray can be approved for clinical trials, its toxicity must be evaluated on cultured human dermal fibroblasts.

Cultured fibroblasts were seeded into four identical culture wells. Sterile phosphate-buffered saline (PBS) was added to the first well as a control, and a different candidate formulation of the antimicrobial spray was added to each of the remaining three wells.

The fibroblasts were incubated for 6 hours6\text{ hours}6 hours, after which the percentage of viable cells was determined.

Table 1 shows the results.

Table 1

Substance added to dermal fibroblastsPercentage of viable cells after 6 hours6\text{ hours}6 hours (%)
Sterile PBS (control)95
Formulation Alpha94
Formulation Beta96
Formulation Gamma95

Explain the conclusion that can be made from this data.

[2]

Antibiotics, culturing microorganisms and monoclonal antibodies Questions

  1. GCSE
  2. /Biology
  3. /Antibiotics, culturing microorganisms and monoclonal antibodies