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6.3 Monitoring and maintaining health

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Question 16

ELISA (enzyme-linked immunosorbent assay) is an essential diagnostic tool used to monitor and maintain health by detecting specific antibodies in a patient's blood serum.

The list below shows five key stages in performing an indirect ELISA test to screen for an infection:

  • A: Enzyme-labeled secondary antibodies are added and bind specifically to the patient's primary antibodies.
  • B: The well is washed thoroughly to remove any unbound enzyme-labeled antibodies.
  • C: The specific antigen of the pathogen is immobilized on the surface of the well.
  • D: A specific substrate is added, which is converted by the enzyme to produce a visible color change.
  • E: The patient's blood serum is added, allowing any primary antibodies present to bind to the antigen.

Write these stages in the correct order in the boxes below. The first one has been done for you.

C \begin{array}{|c|c|c|c|c|} \hline \mathbf{C} & \quad & \quad & \quad & \quad \\ \hline \end{array} C​​​​​​
[4]
Markscheme

6.3 Monitoring and maintaining health Questions

  1. GCSE
  2. /Biology
  3. /6.3 Monitoring and maintaining health

97 exam-style questions on OCR GCSE Biology 6.3 Monitoring and maintaining health, covering 6.3.1 Health and disease, 6.3.2 Interactions between diseases, 6.3.3 Spread of communicable diseases, 6.3.4 Common infections and diseases, 6.3.5 Physical plant defence responses, 6.3.6 Chemical plant defence responses, 6.3.7 Detecting and identifying plant diseases, 6.3.8 White blood cells and platelets, 6.3.9 Non-specific defence systems, 6.3.10 The immune system, 6.3.11 Monoclonal antibodies, 6.3.12 Vaccines and medicines, 6.3.13 Aseptic techniques, 6.3.14 Discovery and development of new medicines, 6.3.15 Causes of non-communicable diseases, 6.3.16 Treatments for cardiovascular disease, 6.3.17 Lifestyle factors and non-communicable diseases, 6.3.18 Cancer, 6.3.19 Stem cells in medicine, 6.3.20 Gene technology in medicine, and 6.3.21 The human genome and medicine. Each one has a worked solution and a mark scheme showing where the marks go.

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