An investigation was carried out into the decomposition of hydrogen peroxide by the enzyme catalase at a temperature of 25∘C25^\circ\text{C}25∘C and a pH of 7.0. The reaction was performed using 10 cm3 of 1.0 mol dm-3 hydrogen peroxide and a fixed concentration of catalase. The volume of oxygen gas produced over time was recorded.
Which modification to the reaction mixture would increase the initial rate of oxygen production, but result in the exact same final total volume of oxygen collected at complete exhaustion of the substrate?
Add a competitive inhibitor of catalase to the reaction mixture
Double the concentration of the catalase enzyme used
Double the concentration of the hydrogen peroxide substrate (keeping the volume constant)
Increase the reaction temperature from 25∘C25^\circ\text{C}25∘C to 80∘C80^\circ\text{C}80∘C
