Scientists studied an enzyme produced by a gene in a species of bacterium.
They found three genetic variants of the gene, V1, V2 and V3. Each variant has a change of one base in the coding DNA of the gene.
The scientists measured the activity of the enzyme produced by each variant.
The Figure shows their results.
Figure

Calculate the percentage decrease in enzyme activity for V3 compared with the normal enzyme.
Suggest why the change in V1 has no effect on the activity of the enzyme.
Explain why the enzyme produced by V3 has very low activity.
44 exam-style questions on Edexcel GCSE Biology 3.2 DNA and protein synthesis, covering 3.2.1 DNA structure as a polymer, 3.2.2 Genome and gene definitions, 3.2.3 Extracting DNA from fruit, 3.2.4 Base order determines protein shape, 3.2.5 Stages of protein synthesis, 3.2.6 Non-coding DNA variants affecting phenotype, and 3.2.7 Coding DNA variants affecting phenotype. Each one has a worked solution and a mark scheme showing where the marks go.