A student is carrying out a biochemical test to detect the presence of sucrose (a non-reducing sugar) in a sample of fruit juice. Which of the following describes the correct sequence of reagents and procedures required to obtain a positive result?
Heat the sample with dilute sodium hydroxide (NaOH\text{NaOH}NaOH), neutralize with hydrochloric acid (HCl\text{HCl}HCl), then add Benedict's reagent and heat.
Heat the sample with dilute hydrochloric acid (HCl\text{HCl}HCl), neutralize with sodium hydrogencarbonate (NaHCO3\text{NaHCO}_3NaHCO3), then add Benedict's reagent and heat.
Add Benedict's reagent to the sample and heat, then add dilute hydrochloric acid (HCl\text{HCl}HCl) and heat again.
Heat the sample with dilute hydrochloric acid (HCl\text{HCl}HCl), neutralize with sodium hydrogencarbonate (NaHCO3\text{NaHCO}_3NaHCO3), then add Biuret reagent.