Practical skills

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12
Question 1
Hard

A student is preparing to observe a stained blood smear slide under a light microscope at high magnification using a 100× 100\times\,100× oil-immersion objective lens.

Which of the following procedures should the student use to safely and correctly focus the specimen?

Focus the specimen under a lower power lens, place a drop of immersion oil directly on the 100×100\times100× objective lens, click the lens into place, and use the coarse adjustment knob to lower the stage while looking through the eyepiece.

Focus the specimen under a lower power lens, rotate the nosepiece halfway, place a drop of immersion oil directly on the slide, click the 100×100\times100× objective lens into position in the oil, and use only the fine adjustment knob to focus.

Place a drop of immersion oil directly on the slide, click the 100×100\times100× objective lens into position, and use the coarse adjustment knob to raise the stage while looking through the eyepiece until the lens is submerged in the oil.

Rotate the 100×100\times100× objective lens into position, place a drop of immersion oil on the lens, raise the stage using the coarse adjustment knob while looking through the eyepiece, and then use the fine adjustment knob to focus.

Practical skills Questions

  1. GCSE
  2. /Biology
  3. /Practical skills