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Question 20

A student is carrying out a biochemical test to detect the presence of sucrose (a non-reducing sugar) in a sample of fruit juice. Which of the following describes the correct sequence of reagents and procedures required to obtain a positive result?

A

Heat the sample with dilute sodium hydroxide (NaOH\text{NaOH}NaOH), neutralize with hydrochloric acid (HCl\text{HCl}HCl), then add Benedict's reagent and heat.

B

Heat the sample with dilute hydrochloric acid (HCl\text{HCl}HCl), neutralize with sodium hydrogencarbonate (NaHCO3\text{NaHCO}_3NaHCO3​), then add Benedict's reagent and heat.

C

Add Benedict's reagent to the sample and heat, then add dilute hydrochloric acid (HCl\text{HCl}HCl) and heat again.

D

Heat the sample with dilute hydrochloric acid (HCl\text{HCl}HCl), neutralize with sodium hydrogencarbonate (NaHCO3\text{NaHCO}_3NaHCO3​), then add Biuret reagent.

Markscheme

Practical skills Questions

  1. GCSE
  2. /Biology
  3. /Practical skills

44 exam-style questions on OCR GCSE Biology Practical skills. Each one has a worked solution and a mark scheme showing where the marks go.

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