A student investigates the effect of temperature on the rate of reaction of a starch-digesting enzyme.
The table shows their results:
| Temperature (∘C^\circ\text{C}∘C) | Rate of reaction (arbitrary units) |
|---|---|
| 10 | 2 |
| 20 | 8 |
| 30 | 22 |
| 40 | 45 |
| 50 | 48 |
| 60 | 5 |
The student wants to increase their confidence in finding the exact optimum temperature for this enzyme.
Which of the following actions should the student take?
Repeat the experiment using temperatures of 42∘C42^\circ\text{C}42∘C, 44∘C44^\circ\text{C}44∘C, 46∘C46^\circ\text{C}46∘C, 48∘C48^\circ\text{C}48∘C, and 50∘C50^\circ\text{C}50∘C.
Repeat the experiment at 10∘C10^\circ\text{C}10∘C intervals up to 100∘C100^\circ\text{C}100∘C.
Repeat the experiment with a higher concentration of the starch substrate.
Repeat the experiment at pH values ranging from 2 to 12.