A student investigates the distribution and structure of stomata on a broad bean leaf.
They prepare a temporary mount of a leaf epidermal peel to view under a light microscope.
Complete each sentence below to describe how they prepare and view the slide. Use words from the box:
coverslipeyepiecefocusforcepsobjectivestagestainwater\begin{array}{|cccc|} \hline \text{coverslip} & \text{eyepiece} & \text{focus} & \text{forceps} \\ \text{objective} & \text{stage} & \text{stain} & \text{water} \\ \hline \end{array}coverslipobjectiveeyepiecestagefocusstainforcepswater
- To obtain a thin specimen, the student uses ____________________ to peel a single layer of cells from the leaf surface.
- The peel is placed onto a clean glass slide in a drop of ____________________ to keep the cells hydrated.
- A ____________________ is lowered carefully at an angle onto the slide to avoid trapping air bubbles.
- The prepared slide is then positioned securely on the flat microscope ____________________ before being focused.
The diagram below represents a region of the epidermal peel observed under high power.
State the name of the specialised cell labelled A.
State the name of the organelle labelled B which is visible as a distinct circular structure inside cell A.
Give one reason why the sample of tissue was taken from the lower epidermis of the leaf rather than the upper epidermis.