A technician is making recombinant DNA using a gene from a fungus and a bacterial plasmid. Two prepared gene-plasmid combinations are available.
The bases in each row are already shown in their pairing positions: the first base in the gene sequence would meet the first base in the plasmid sequence.
| Combination | Gene sticky end | Plasmid sticky end |
|---|---|---|
| P | A A G C | T T C G |
| Q | T T A C | C A G T |
Which combination should the technician use? Explain how the gene becomes permanently joined to the plasmid.
127 exam-style questions on OCR GCSE Biology 6.2 Feeding the human race, covering 6.2.1 Biological factors affecting food security, 6.2.2 Agricultural solutions to growing demand, 6.2.3 Selective breeding, 6.2.4 Genetic engineering, 6.2.5 Steps in genetic engineering, 6.2.6 Benefits and risks of gene technology in agriculture, and 6.2.7 Biotechnological solutions to growing demand. Each one has a worked solution and a mark scheme showing where the marks go.