A researcher wants to insert a gene from a marine alga into a bacterial plasmid. Enzyme A cuts DNA at the sequence GATC, which occurs at the intended cut site in both the gene and the plasmid. Enzyme B cuts DNA at the sequence CCGG, which occurs in the gene but not in the plasmid.
Which statement explains which enzyme the researcher should use to cut the gene and plasmid?
Use enzyme B on the gene and enzyme A on the plasmid, because both enzymes leave sticky ends.
Use enzyme A on the gene and enzyme B on the plasmid, because each enzyme cuts DNA at a specific sequence.
Use enzyme B on both, because cutting the gene at CCGG is enough for it to fit into the plasmid.
Use enzyme A on both, because it cuts at GATC in each DNA molecule, leaving matching sticky ends.
127 exam-style questions on OCR GCSE Biology 6.2 Feeding the human race, covering 6.2.1 Biological factors affecting food security, 6.2.2 Agricultural solutions to growing demand, 6.2.3 Selective breeding, 6.2.4 Genetic engineering, 6.2.5 Steps in genetic engineering, 6.2.6 Benefits and risks of gene technology in agriculture, and 6.2.7 Biotechnological solutions to growing demand. Each one has a worked solution and a mark scheme showing where the marks go.