A conservation centre uses tissue culture to produce clones of a rare tree fern. A sterile explant has formed a mass of cells on nutrient agar.
Which sequence should be used next?
Transfer small groups of cells to media with suitable hormone balances; allow shoots and roots to develop; move the plantlets to compost.
Allow shoots and roots to develop throughout the cell mass; move the plantlets to compost; separate them onto hormone media.
Transfer small groups of cells to compost; allow shoots to develop; return the plantlets to nutrient agar so that roots develop.
Move the cell mass to compost; allow shoots and roots to develop; transfer the plantlets to media with suitable hormone balances.
100 exam-style questions on Edexcel GCSE Biology 4.3 Selective breeding and genetic modification, covering 4.3.1 Selective breeding and its impact, 4.3.2 Tissue culture, 4.3.3 Genetic engineering overview, 4.3.4 Main stages of genetic engineering, 4.3.5 Advantages and disadvantages of GM organisms, 4.3.6 Agricultural solutions for a growing population, and 4.3.7 Evaluating genetic engineering and breeding. Each one has a worked solution and a mark scheme showing where the marks go.