Use of apparatus and techniques

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Question 3
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An environmental scientist carries out a serial dilution of a water sample from a local lake to estimate the population of a specific green alga (Chlorella).

The scientist uses the following method:

  • Transfer 5 cm35\text{ cm}^35 cm3 of the lake water from an original sample of 120 cm3120\text{ cm}^3120 cm3 to a clean beaker.
  • Carry out four 5-fold serial dilutions. Each dilution involves transferring 2 cm32\text{ cm}^32 cm3 of the solution to a test tube containing 8 cm38\text{ cm}^38 cm3 of sterile distilled water.
  • Transfer 0.2 cm30.2\text{ cm}^30.2 cm3 of the final diluted solution to a microscope counting chamber.
  • Count the algal cells under a microscope.

The scientist counts 48 algal cells in the counting chamber.

Calculate the total number of algal cells in the original 120 cm3120\text{ cm}^3120 cm3 lake water sample. Assume the cells are distributed uniformly and that each counted cell represents a single individual in the diluted volume. Give your answer in standard form.

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Use of apparatus and techniques Questions

  1. A Level
  2. /Biology
  3. /Use of apparatus and techniques