Question 1

A light micrograph of a pollen grain was taken using a $×10\times 10$ objective lens. The eyepiece graticule was calibrated using a stage micrometer.

The 0 mark on the eyepiece graticule aligns with the 0 mark on the stage micrometer.
The 80 mark on the eyepiece graticule aligns with the 7th division mark on the stage micrometer scale.

Each division on the stage micrometer scale represents exactly $mm0.01\text{ mm}$ .

The pollen grain is measured using the same eyepiece graticule and is found to span from division 8 to division 43 on the eyepiece graticule scale.

Calculate the actual width of the pollen grain. Give your answer in $μm\mu\text{m}$ to 2 significant figures.

A light micrograph of a pollen grain was taken using a $×10\times 10$ objective lens. The eyepiece graticule was calibrated using a stage micrometer.

The 0 mark on the eyepiece graticule aligns with the 0 mark on the stage micrometer.
The 80 mark on the eyepiece graticule aligns with the 7th division mark on the stage micrometer scale.

Each division on the stage micrometer scale represents exactly $mm0.01\text{ mm}$ .

The pollen grain is measured using the same eyepiece graticule and is found to span from division 8 to division 43 on the eyepiece graticule scale.

Calculate the actual width of the pollen grain. Give your answer in $μm\mu\text{m}$ to 2 significant figures.

Use of apparatus and techniques