Which statement correctly describes how a chemical or physical change affects the specific bonds holding a single-chain protein's structure together?
A reducing agent specifically cleaves the hydrogen bonds along the polypeptide backbone, directly destabilising α\alphaα-helices.
An extreme shift in pH alters the ionization of charged R-groups, disrupting ionic and hydrogen bonds, thereby destabilising the tertiary structure.
An increase in temperature to 50 ∘C50\ ^\circ\text{C}50 ∘C provides sufficient thermal energy to hydrolyse peptide bonds, breaking down the primary structure.
Hydrophobic interactions, which stabilise the secondary structure, are disrupted by organic solvents, leading to the collapse of β\betaβ-pleated sheets.
