Antibiotics, culturing microorganisms and monoclonal antibodies

An investigator is preparing a culture of E. coli bacteria. Below is a list of the first two steps in their method:

Step 1: Wipe the work surface with disinfectant.
Step 2: Heat the metal inoculating loop in a Bunsen burner flame until it glows red.

State why both Step 1 and Step 2 are necessary before transferring the bacteria to the nutrient agar plate.

[1]

Antibiotics, culturing microorganisms and monoclonal antibodies Questions

An investigator is preparing a culture of E. coli bacteria. Below is a list of the first two steps in their method:

Step 1: Wipe the work surface with disinfectant.
Step 2: Heat the metal inoculating loop in a Bunsen burner flame until it glows red.

State why both Step 1 and Step 2 are necessary before transferring the bacteria to the nutrient agar plate.

[1]