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Nucleotides and nucleic acids

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Question 1
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A parent DNA molecule with both strands labeled with light nitrogen (14N^{14}\text{N}14N) is transferred to a growth medium containing only heavy nitrogen (15N^{15}\text{N}15N) as the sole nitrogen source, and allowed to undergo three rounds of semiconservative replication.

Which of the following statements correctly predict the results?

  1. After one round of replication, 100% of the DNA molecules are hybrid (14N/15N^{14}\text{N}/^{15}\text{N}14N/15N).
  2. After two rounds of replication, 50% of the DNA strands present in the population contain 14N^{14}\text{N}14N.
  3. After three rounds of replication, the ratio of heavy-density DNA molecules (15N/15N^{15}\text{N}/^{15}\text{N}15N/15N) to intermediate-density DNA molecules (14N/15N^{14}\text{N}/^{15}\text{N}14N/15N) is 3:13:13:1.

1 and 2 only

1 and 3 only

2 and 3 only

1, 2 and 3

Nucleotides and nucleic acids Questions

  1. A Level
  2. /Biology
  3. /Nucleotides and nucleic acids