A researcher aims to express a human polypeptide in the bacterium Escherichia coli using in vivo cloning techniques.
Which of the following statements correctly explains a requirement or outcome of this procedure?
The human gene can be extracted directly from genomic DNA using restriction endonucleases because E. coli possesses the spliceosomes necessary to remove human introns.
Reverse transcriptase is used to synthesize complementary DNA (cDNAcDNAcDNA) from mature mRNA, ensuring the gene insert lacks introns so a functional polypeptide can be translated.
DNA ligase is used to cleave both the human cDNAcDNAcDNA and the bacterial plasmid vector at specific palindromic recognition sequences to generate complementary sticky ends.
A eukaryotic promoter sequence must be inserted upstream of the cloned gene to allow bacterial RNA polymerase to initiate transcription of the human gene.
