This question is about paper chromatography.
A student wants to analyze the photosynthetic pigments in a sample of green leaf extract.
Plan an investigation to determine the RfR_fRf value of the main yellow-green pigment in this leaf extract.
Your plan should describe how to use:
- a chromatography paper strip
- a beaker with a lid
- a suitable solvent (such as ethanol)
- a capillary tube containing the green leaf extract
Include the measurements required and the formula used to calculate the final RfR_fRf value.
Two students performed paper chromatography on the same green leaf extract, but obtained different RfR_fRf values for the yellow-green pigment.
Which of the following differences in their investigations would result in different RfR_fRf values?
- Using a wider beaker
- Leaving the chromatography paper in the beaker for a longer duration
- Using a different solvent
- Using a longer strip of chromatography paper
In paper chromatography, a solvent rises up the paper to separate the components of a mixture.
What is the mobile phase in this process?
- Beaker
- Leaf extract
- Paper
- Solvent