Purity, formulations and chromatography

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Question 2
Medium

A biochemist analyzed a spinach leaf extract containing carotene and lutein pigments using two different chromatography stationary phases: Matrix A and Matrix B.

The table below shows the results of the analysis:

Stationary PhasePigmentDistance moved by pigment (cm\text{cm}cm)Distance moved by solvent (cm\text{cm}cm)RfR_fRf​ value
Matrix ACarotene12.016.00.75
Matrix ALutein4.016.00.25
Matrix BCaroteneddd16.00.70
Matrix BLutein5.616.00.35
a.

Calculate the distance moved by the carotene pigment on Matrix B, represented by ddd.

[2]
b.

Explain which stationary phase (Matrix A or Matrix B) has a stronger attraction to lutein, using the RfR_fRf​ values from the table.

[3]

Purity, formulations and chromatography Questions

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  2. /Chemistry
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