Purity, formulations and chromatography

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Question 13
Medium

A student investigated a dye mixture, M\text{M}M, and four reference dyes, W\text{W}W, X\text{X}X, Y\text{Y}Y, and Z\text{Z}Z, using paper chromatography.

The resulting chromatogram is shown below:

A chromatogram on chromatography paper. There is a horizontal start line at the bottom and a solvent front line at the top. The distance from the start line to the solvent front is 8.0 cm. There are five lanes labeled W, X, Y, Z, and M. Lane W has one spot at 2.0 cm above the start line. Lane X has one spot at 3.6 cm above the start line. Lane Y has one spot at 6.4 cm above the start line. Lane Z has one spot remaining directly on the start line. Lane M has two spots: one at 3.6 cm and one at 5.2 cm above the start line.

a.

Which of the four reference dyes is present in mixture M\text{M}M?

[2]
b.

Calculate the RfR_{\text{f}}Rf​ value of this reference dye using the equation:

Rf=distance moved by dyedistance moved by solventR_{\text{f}} = \frac{\text{distance moved by dye}}{\text{distance moved by solvent}}Rf​=distance moved by solventdistance moved by dye​

[3]

Purity, formulations and chromatography Questions

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