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Antibiotics, culturing microorganisms and monoclonal antibodies

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Question 2

An agricultural researcher is investigating the antimicrobial efficacy of three organic essential oils—Oregano oil, Tea Tree oil, and Lemongrass oil—against the plant pathogen Pseudomonas syringae.

The researcher is provided with:

  • a prepared agar plate, already inoculated with a uniform lawn of Pseudomonas syringae
  • bottles containing pure Oregano, Tea Tree, and Lemongrass oils
  • sterile filter paper discs
  • sterile forceps
  • sterile water
  • adhesive tape
  • a ruler.

Figure 1 shows the final appearance of the agar plate after the discs have been added and the plate has been incubated.

The final appearance of the agar plate showing four paper discs labeled O (Oregano oil), T (Tea Tree oil), L (Lemongrass oil), and C (Control) with clear zones of inhibition around O, T, and L, and no zone around C.

Figure 1

Devise a laboratory protocol for the researcher to carry out this investigation to obtain the results shown in Figure 1. Include details of a suitable control, the variables that must be kept constant, and how the effectiveness of the oils should be measured and compared.

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Antibiotics, culturing microorganisms and monoclonal antibodies Questions

  1. GCSE
  2. /Biology
  3. /Antibiotics, culturing microorganisms and monoclonal antibodies