An agricultural researcher is investigating the antimicrobial efficacy of three organic essential oils—Oregano oil, Tea Tree oil, and Lemongrass oil—against the plant pathogen Pseudomonas syringae.
The researcher is provided with:
Figure 1 shows the final appearance of the agar plate after the discs have been added and the plate has been incubated.

Figure 1
Devise a laboratory protocol for the researcher to carry out this investigation to obtain the results shown in Figure 1. Include details of a suitable control, the variables that must be kept constant, and how the effectiveness of the oils should be measured and compared.