Students are investigating the effect of temperature on the rate of respiration in yeast. They measured the rate of carbon dioxide production (in cm3 min−1\text{cm}^3\,\text{min}^{-1}cm3min−1) with multiple replicates at 25∘C25^\circ\text{C}25∘C and 35∘C35^\circ\text{C}35∘C. The students want to determine whether the mean rate of carbon dioxide production is significantly greater at 35∘C35^\circ\text{C}35∘C compared to 25∘C25^\circ\text{C}25∘C.
Which statistical test should they use?
Chi-squared (χ2\chi^2χ2) test
Student's ttt-test
Spearman's rank correlation coefficient
Standard deviation