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Cloning and biotechnology

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Question 14

A forensic scientist is performing genetic fingerprinting to analyze variable number tandem repeats (VNTRs) from a genomic DNA sample. Which of the following options shows the correct sequence of steps for this analysis?

Digest DNA with restriction endonucleases →\rightarrow→ Separate fragments by gel electrophoresis →\rightarrow→ Transfer fragments to a nylon membrane →\rightarrow→ Hybridise with a labelled DNA probe →\rightarrow→ Detect bands using autoradiography

Separate genomic DNA by gel electrophoresis →\rightarrow→ Digest DNA in the gel with restriction endonucleases →\rightarrow→ Transfer fragments to a nylon membrane →\rightarrow→ Hybridise with a labelled DNA probe →\rightarrow→ Detect bands using autoradiography

Digest DNA with restriction endonucleases →\rightarrow→ Hybridise with a labelled DNA probe in solution →\rightarrow→ Separate fragments by gel electrophoresis →\rightarrow→ Transfer fragments to a nylon membrane →\rightarrow→ Detect bands using autoradiography

Digest DNA with restriction endonucleases →\rightarrow→ Transfer genomic DNA to a nylon membrane →\rightarrow→ Separate fragments on the membrane using electrophoresis →\rightarrow→ Hybridise with a labelled DNA probe →\rightarrow→ Detect bands using autoradiography

Cloning and biotechnology Questions

  1. A Level
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