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3b:T438,- **Change (C):** Vary the independent variable (temperature) using water baths at a range of at least 5 different temperatures (e.g. $20^\circ\text{C}$, $30^\circ\text{C}$, $40^\circ\text{C}$, $50^\circ\text{C}$, and $60^\circ\text{C}$) B1 - **Organism (O):** Control the biological material by using the same species of yeast and keeping the volume and concentration of the yeast suspension constant B1 - **Repeat (R):** Repeat the experiment at least three times at each temperature to enable the calculation of a reliable mean B1 - **Measure 1 (M1):** Measure the volume of carbon dioxide gas produced (using a gas syringe) or count the bubbles of gas released B1 - **Measure 2 (M2):** Measure this gas production over a set/specified time period (e.g. 5 or 10 minutes) to determine the rate B1 - **Standardise (S1 & S2):** Control at least two other key variables, such as keeping the concentration and volume of glucose solution constant, and keeping the pH constant using a buffer 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Respiration

Question 3

Respiration Questions

Respiration

Question 3

Respiration Questions