In plant micropropagation (a method of asexual reproduction), explants (small pieces of plant tissue) must be surface-sterilised using a solution of sodium hypochlorite (bleach) before they are cultured on sterile nutrient agar. This sterilisation process kills fungal spores and bacterial cells on the tissue surface, but if the concentration of sodium hypochlorite is too high, it may damage the plant cells and prevent shoot regeneration.
Design an investigation to find the optimal concentration of sodium hypochlorite to sterilise the explants while allowing maximum shoot regeneration.
Your answer should include experimental details and be written in full sentences.
