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Question 6

In commercial sweet cherry (Prunus avium) orchards, hand pollination is increasingly used to maximize crop yield. For successful fertilization, germinating pollen grains must rapidly grow a pollen tube down the style to deliver the male gametes to the ovule. The pH of the stigmatic fluid can vary depending on environmental factors.

Design an investigation to find the optimum pH of an in vitro growth medium for the rate of pollen tube growth in Prunus avium pollen grains.

Your answer should include experimental details and be written in full sentences.

[6]

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