- How scientists investigate the distribution and abundance of organisms in a habitat.
- How to use quadrats, transects and capture-recapture to estimate population size.
- How human activities can damage or protect biodiversity.
- How to judge evidence linking environmental changes to where organisms live.
An ecosystem is a community of organisms interacting with each other and with the non-living environment. Ecosystems are not fixed: they change when temperature, water, food supply, pollution, land use or hunting pressure changes.
Monitoring means collecting data over time so we can spot patterns, such as a species becoming rarer, moving into a new area, or recovering after conservation work.
Core ecology words
- A habitat is the place where an organism lives.
- A population is all the organisms of one species in a particular area.
- A community is all the populations of different species living together.
- Distribution means where organisms are found.
- Abundance means how many organisms there are.
- Biodiversity is the variety of living organisms in an area, including different species and genetic variation.
A community is affected by biotic factors, which are living factors such as predators, disease, competition and food, and abiotic factors, which are non-living conditions such as light intensity, temperature, water availability, pH and gases in the air or water.
In a large habitat, it is usually impossible to count every organism. Instead, ecologists take a sample, which is a smaller part of the habitat used to estimate what is happening in the whole area.
A good sample should be representative, meaning it reflects the habitat fairly. Scientists use repeat samples, calculate a mean, and avoid biased choices such as only sampling the easiest places to reach.
Good sampling
A good ecological investigation uses a clear method, enough repeats, and a fair way of choosing sampling sites so the results can be scaled up to estimate the whole habitat.
A quadrat is a square frame placed on the ground to sample organisms in a known area. Quadrats are useful for plants and slow-moving animals, such as limpets or snails.
For random sampling, you might:
- Mark out the study area using tape measures.
- Use a grid and random coordinates to choose quadrat positions.
- Place the quadrat at each coordinate.
- Count the number of organisms, or estimate percentage cover.
- Calculate a mean and scale up to the total area.
This diagram compares random quadrat sampling with transect sampling, which you’ll meet next.

The scaling-up idea is:
estimated population=mean per quadrat×total habitat areaarea of one quadrat\text{estimated population}=\text{mean per quadrat}\times \frac{\text{total habitat area}}{\text{area of one quadrat}}estimated population=mean per quadrat×area of one quadrattotal habitat area
Estimating daisies with quadrats
A student uses five 1 m² quadrats in a 200 m² lawn. The daisy counts are 8, 12, 10, 6 and 14.
- Calculate the mean number of daisies per quadrat: 8+12+10+6+145=10\frac{8+12+10+6+14}{5}=1058+12+10+6+14=10 daisies per quadrat.
- Convert this into a density: because each quadrat is 1 m², the estimate is 10 daisies per m².
- Scale up to the whole lawn: 10×200=200010 \times 200=200010×200=2000, so the estimated population is 2000 daisies.
Choosing nice-looking quadrat sites
Do not place quadrats only where there are lots of organisms. That gives biased results. Random coordinates make the sample fairer.
A transect is a line across a habitat. Transects are useful when you expect conditions to change gradually, such as from a shaded woodland edge into open grassland.
In a line transect, you record organisms touching the tape. In a belt transect, you place quadrats at regular intervals along the tape and count organisms within each quadrat.
Transects are especially good for showing changes in distribution, not just total abundance. For example, moss may be common in shaded damp areas but less common in bright dry areas.
A sensible graph might show distance along the transect on the x-axis and number of organisms on the y-axis. If the x-axis is continuous data, such as distance in metres, a line graph or scatter graph is often suitable.
Ecologists also use:
- Keys: step-by-step identification guides based on features of organisms.
- Pooters: small devices used to collect tiny invertebrates safely.
- Nets: sweep nets for insects in long grass, or pond nets for aquatic animals.
The method you choose depends on the organism. Plants do not run away, so quadrats work well. Flying insects or pond animals usually need nets or traps.
Capture-recapture, also called mark-release-recapture, estimates the population size of mobile animals.
The basic method is:
- Capture a first sample.
- Mark them safely.
- Release them and allow time for them to mix back into the population.
- Capture a second sample.
- Count how many in the second sample are already marked.

The formula is:
N=n1×n2RN=\frac{n_1 \times n_2}{R}N=Rn1×n2
where NNN is the estimated population size, n1n_1n1 is the number caught first, n2n_2n2 is the number caught second, and RRR is the number of marked animals recaptured in the second sample.
Estimating a woodlouse population
A student catches and marks 40 woodlice. Later, they catch 50 woodlice, and 10 of these are marked.
- Identify the values: n1=40n_1=40n1=40, n2=50n_2=50n2=50, and R=10R=10R=10.
- Substitute into the formula: N=40×5010=200N=\frac{40 \times 50}{10}=200N=1040×50=200.
- Interpret the result: the estimated population is about 200 woodlice, assuming the marked woodlice mixed evenly with the rest of the population.
Capture-recapture assumptions
Capture-recapture is unreliable if marks fall off, the mark affects survival, animals do not mix randomly, or the population is not closed. A closed population has no major births, deaths, immigration or emigration during the investigation.
Humans can affect ecosystems negatively or positively. It is important not to assume every human interaction is harmful.
Negative interactions include:
- Land use changes, such as deforestation, farming, road building and housing, which destroy or fragment habitats.
- Hunting, poaching and overfishing, which can remove organisms faster than they reproduce.
- Pollution, including air pollution, water pollution and acid rain.
- Introduction of invasive species, which may outcompete native species.
Positive interactions include:
- Protecting individual species using laws, breeding programmes and anti-poaching work.
- Conserving selected habitats, such as woodlands, wetlands, coral reefs or wildflower meadows.
- Restoring damaged habitats by planting native species or creating wildlife corridors.
- Reducing pollution and managing resources sustainably.
Predicting the effect of land use
A woodland is cleared and replaced by a car park.
- Identify the direct change: the number of trees and plant species decreases, so there are fewer habitats and food sources.
- Link this to animals: fewer nesting sites, shelter and food sources may reduce bird, insect and mammal populations.
- Predict the biodiversity impact: biodiversity is likely to decrease locally because fewer species can survive in the changed habitat.
Only writing “humans are bad”
GCSE answers often need both sides. Humans can reduce biodiversity through habitat destruction, but conservation schemes can protect species and restore habitats.
Maintaining biodiversity has many benefits. Diverse ecosystems are often more stable because if one species declines, others may still carry out similar roles. Biodiversity also supports pollination, soil formation, clean water, medicines, food security and genetic resources for future crops.
Ecotourism means tourism based on wildlife or natural habitats. If managed carefully, it can provide jobs and money for conservation while encouraging local people to protect ecosystems.
But conservation is not simple. Challenges include:
- Cost of monitoring and protecting habitats.
- Conflict between conservation and farming, housing, mining or transport.
- Difficulty getting agreements between countries for global issues.
- Illegal hunting and wildlife trade.
- Climate change affecting habitats across national borders.
- Tourism disturbing wildlife if visitor numbers are not controlled.
This next skill is Higher Tier only, and for OCR Gateway it is in separate Biology J247 rather than Combined Science.
Environmental changes can affect where organisms are found. For example, water conditions such as pH, temperature, dissolved oxygen or pollutants can affect aquatic life. Atmospheric gases can also matter: increased carbon dioxide is linked with climate change, while sulfur dioxide pollution can affect lichens and contribute to acid rain.
When you evaluate evidence, ask whether the data really supports the conclusion. Look for sample size, repeats, controls, a sensible sampling method, and whether other variables could explain the pattern.
A percentile tells you where a value sits within a data set. For example, the 90th percentile is higher than about 90% of the readings. Percentiles can help compare pollution levels or biodiversity data between sites.
Correlation is not automatically causation
If two things change together, that is a correlation. To claim one caused the other, you need stronger evidence, such as repeated data, a plausible biological explanation, and control of other factors.
Comparing lichen data with sulfur dioxide
Two areas are sampled for lichen species. Four sites with low sulfur dioxide have 8, 7, 9 and 8 species. Four sites with high sulfur dioxide have 2, 1, 3 and 2 species.
- Calculate the mean for the low sulfur dioxide sites: 8+7+9+84=8\frac{8+7+9+8}{4}=848+7+9+8=8 species.
- Calculate the mean for the high sulfur dioxide sites: 2+1+3+24=2\frac{2+1+3+2}{4}=242+1+3+2=2 species.
- Compare and evaluate: the data supports a link between higher sulfur dioxide and fewer lichen species, but you should check other factors such as tree species, light, moisture and sampling method before claiming sulfur dioxide is the only cause.
In the exam
- For sampling questions, name the method, explain why it suits the organism, and include repeats, a mean and scaling up where relevant.
- For calculations, write the formula, substitute the values, and check that your estimate is sensible for the area sampled.
- For biodiversity questions, link cause to effect: human action → habitat or population change → impact on biodiversity.
Check yourself
- How would you estimate the number of daisies in a 500 m² field using quadrats?
- Why might capture-recapture give an unreliable estimate?
- Give one positive and one negative human interaction within an ecosystem, and explain the effect on biodiversity.