A scientist wants to genetically engineer bacteria to produce protein Z. The gene for protein Z is taken from another organism.
The scientist considers four procedures.
| Procedure | Restriction enzyme used to cut out the gene | Restriction enzyme used to open the plasmid | DNA ligase added | Plasmid mixed with bacteria |
|---|---|---|---|---|
| A | 1 | 1 | Yes | Yes |
| B | 1 | 2 | Yes | Yes |
| C | 1 | 1 | No | Yes |
| D | 1 | 1 | Yes | No |
Explain which procedure is most likely to produce bacteria that make protein Z.
80 exam-style questions on AQA GCSE Biology 6.2 Variation and evolution, covering 6.2.1 Variation, 6.2.2 Evolution, 6.2.3 Selective breeding, 6.2.4 Genetic engineering, and 6.2.5 Cloning. Each one has a worked solution and a mark scheme showing where the marks go.