A student investigated the effect of sucrose concentration on anaerobic respiration in yeast. The student used the following method:
- Add 15 cm3 of yeast and water mixture to each of four fermentation tubes connected to gas syringes.
- Add sucrose to each mixture to create concentrations of 0%, 1%, 2%, and 3%.
- Place the setups in a water bath at 35∘C35^\circ\text{C}35∘C for 20 minutes.
- Record the final total volume reading in each gas syringe.
At the start of the investigation, the initial gas volume in each syringe was 5 cm3 (due to dead space in the tubing).
The diagram below shows the gas syringes at the end of the investigation:
The table below shows the results:
| Sucrose concentration | Total gas syringe reading in cm3\text{cm}^3cm3 |
|---|---|
| 0% | 5 |
| 1% | 17 |
| 2% | VVV |
| 3% | 67 |
a.[1]
Find the value of V V\,V from the diagram.
b.[2]
Calculate the volume of carbon dioxide gas produced by the yeast in the syringe with 2% sucrose.