An investigator researched the gravitropic response of young maize (Zea mays) coleoptiles.
Ten coleoptiles were pinned horizontally to a cork backing in each of two separate environmental chambers:
Both chambers were maintained at a constant temperature of 22∘C22^\circ\text{C}22∘C in total darkness for 36 hours36\text{ hours}36 hours.
Explain why it was essential to conduct the entire experiment in total darkness.
Explain the purpose of mounting Chamber B on a slowly rotating clinostat.
Describe how the orientation of the coleoptiles in Chamber A will differ from those in Chamber B after 36 hours36\text{ hours}36 hours.
Explain how the plant hormone indoleacetic acid (IAA) causes the specific growth response observed in Chamber A.