3.2 Monoclonal antibodies (biology only) (HT only)

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Question 1
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A diagnostic lateral flow assay is used to screen blood serum samples for the presence of prostate-specific antigen (PSA) using monoclonal antibodies.

The mechanics of the test strip are as follows:

  • The sample is applied to the end of the strip and moves upwards via capillary action.
  • The sample pad contains mobile, dye-conjugated monoclonal antibodies specific to PSA.
  • The test zone (TTT) contains immobilized monoclonal antibodies that bind specifically to a different site on the PSA molecule.
  • The control zone (CCC) contains immobilized antibodies that bind specifically to the mobile dye-conjugated antibodies to confirm successful fluid migration.

Lateral Flow Assay Strips

Which test strip represents a valid negative result, confirming that PSA is below the threshold of detection and that the assay has run correctly?

Strip W

Strip X

Strip Y

Strip Z

3.2 Monoclonal antibodies (biology only) (HT only) Questions

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