1.1 Cell structure

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Question 29
Medium

A student wants to estimate the concentration of bacteria in a liquid culture using a counting chamber under a microscope. The table below shows the number of bacteria counted in four separate samples using the counting chamber:

SampleNumber of bacteria in counting chamber
122
218
325
415

The dimensions of the counting chamber are:

  • Width: 0.2 mm0.2\text{ mm}0.2 mm
  • Length: 0.2 mm0.2\text{ mm}0.2 mm
  • Depth: 0.02 mm0.02\text{ mm}0.02 mm
a.

Calculate the mean number of bacteria in the four samples.

[1]
b.

Calculate the total area of the counting chamber in mm2\text{mm}^2mm2.

[1]
c.

Calculate the volume of the counting chamber in mm3\text{mm}^3mm3 using the equation: volume=area×depth\text{volume} = \text{area} \times \text{depth}volume=area×depth

[1]
d.

Calculate the mean number of bacteria per mm3\text{mm}^3mm3 of the culture using the equation: mean number of bacteria per mm3=mean number of bacteriavolume of counting chamber\text{mean number of bacteria per }\text{mm}^3 = \frac{\text{mean number of bacteria}}{\text{volume of counting chamber}}mean number of bacteria per mm3=volume of counting chambermean number of bacteria​

[2]

1.1 Cell structure Questions

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