A microbiologist is estimating the concentration of yeast cells in a suspension culture using a hemocytometer grid under a light microscope. The table below shows the number of yeast cells counted in four separate grid zones:
| Zone | Number of yeast cells |
|---|---|
| A | 34 |
| B | 29 |
| C | 31 |
| D | 26 |
The dimensions of each grid zone on the hemocytometer are:
Calculate the mean number of yeast cells in the four zones.
Calculate the total area of one grid zone in mm2\text{mm}^2mm2.
Calculate the volume of one grid zone in mm3\text{mm}^3mm3 using the equation: volume=area×depth\text{volume} = \text{area} \times \text{depth}volume=area×depth
Calculate the mean number of yeast cells per mm3\text{mm}^3mm3 of the suspension culture using the equation: mean number of yeast cells per mm3=mean number of yeast cellsvolume of grid zone\text{mean number of yeast cells per }\text{mm}^3 = \frac{\text{mean number of yeast cells}}{\text{volume of grid zone}}mean number of yeast cells per mm3=volume of grid zonemean number of yeast cells