Untreated sewage is released into a river at one point. A scientist counted the number of invertebrate species living at five sites downstream of this point.
Figure: The number of invertebrate species found at five sites at different distances downstream of a sewage outflow

Use the figure. Describe how the number of invertebrate species changes with distance downstream of the sewage outflow. Use data in your answer.
Suggest why the number of invertebrate species is lowest at the site nearest to the sewage outflow.
The scientist concluded that the sewage caused the low number of species at 1 km. Give one further piece of evidence the scientist would need to support this conclusion.
49 exam-style questions on AQA GCSE Biology 7.3 Biodiversity and the effect of human interaction on ecosystems, covering 7.3.1 Biodiversity, 7.3.2 Waste management, 7.3.3 Land use, 7.3.4 Deforestation, 7.3.5 Global warming, and 7.3.6 Maintaining biodiversity. Each one has a worked solution and a mark scheme showing where the marks go.