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Foundations in biology

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Question 11

A parent DNA molecule with both strands labeled with light nitrogen (14N^{14}\text{N}14N) is transferred to a growth medium containing only heavy nitrogen (15N^{15}\text{N}15N) and allowed to undergo four rounds of replication.

Which of the following statements correctly predict the results if DNA replication is semiconservative?

  1. After three rounds of replication, 75% of the double-stranded DNA molecules are of heavy density (15N/15N^{15}\text{N}/^{15}\text{N}15N/15N).
  2. After four rounds of replication, the ratio of hybrid-density (14N/15N^{14}\text{N}/^{15}\text{N}14N/15N) to heavy-density (15N/15N^{15}\text{N}/^{15}\text{N}15N/15N) DNA molecules is 1:81:81:8.
  3. After three rounds of replication, 12.5% of the individual polynucleotide strands in the population contain 14N^{14}\text{N}14N.

1 and 2 only

1 and 3 only

2 and 3 only

1, 2 and 3

Foundations in biology Questions

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