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Foundations in biology

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Question 9

A parent DNA molecule with both strands labeled with heavy nitrogen (15N^{15}\text{N}15N) is transferred to a growth medium containing only light nitrogen (14N^{14}\text{N}14N) and allowed to undergo three rounds of replication.

Which of the following statements correctly predict the results if DNA replication is semiconservative?

  1. After one round of replication, 100% of the DNA molecules have an intermediate hybrid density (15N/14N^{15}\text{N}/^{14}\text{N}15N/14N).
  2. After two rounds of replication, there is an equal proportion of intermediate density (15N/14N^{15}\text{N}/^{14}\text{N}15N/14N) molecules and light density (14N/14N^{14}\text{N}/^{14}\text{N}14N/14N) molecules.
  3. After three rounds of replication, 75% of the DNA molecules have intermediate hybrid density (15N/14N^{15}\text{N}/^{14}\text{N}15N/14N) and 25% have light density (14N/14N^{14}\text{N}/^{14}\text{N}14N/14N).

only 1 and 2

only 1 and 3

only 2 and 3

1, 2 and 3

Foundations in biology Questions

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